Proteintech, 25474-1-AP, CTGF Polyclonal antibody

Size: 20ul / 150ul The CTGF (25474-1-AP) by Proteintech is a Polyclonal antibody targeting CTGF in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 25474-1-AP targets CTGF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HUVEC cells, HeLa cells, MG-63 cells, U2OS cells Positive IP detected in: HUVEC cells Positive IHC detected in: human ovary tumor tissue, mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, canine, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: connective tissue growth factor Calculated Molecular Weight: 38 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: NM_001901 Gene Symbol: CTGF Gene ID (NCBI): 1490 RRID: AB_2918089 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P29279 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.