Proteintech, 25486-1-AP, CKAP2 Polyclonal antibody

Size: 20ul / 150ul The CKAP2 (25486-1-AP) by Proteintech is a Polyclonal antibody targeting CKAP2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 25486-1-AP targets CKAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, PC-3 cells, SGC-7901 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Cytoskeleton-associated protein 2 (CKAP2), also known as tumor-associated microtubule-associated protein (TMAP), is implicated in the regulation of cell proliferation (PMID: 30119212). CKAP2 is a MAP expressed during mitosis that localizes to centrosomes and spindles before being targeted for degradation by the anaphase-promoting complex/cyclosome at mitotic exit (PMID: 38381793, 17339342). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21901 Product name: Recombinant human CKAP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-356 aa of BC136332 Sequence: MSTPAVPQDLQLPPSQRAQSAFKEQRRQKLKEHLLRRKTLFAYKQENEMLSSRDQRVVTSEDQVQEGTKVLKLKTKMADKENMKRPAESKNNTVVGKHCIPLKPSNELTNSTVVIDTHKPKDSNQTPHLLLTEDDPQSQHMTLSQAFHLKNNSKKKQMTTEKQKQDANMPKKPVLGSYRGQIVQSKINSFRKPLQVKDESSAATKKLSATIPKATKPQPVNTSSVTVKSNRSSNMTATTKFVSTTSQNTQLVRPPIRSHHSNTRDTVKQGISRTSANVTIRKGPHEKELLQSKTALSSVKTSSSQGIIRNKTLSRSIASEVVARPASLSNDKLMEKSEPVDQRRHTAGKAIVDSRS Predict reactive species Full Name: cytoskeleton associated protein 2 Calculated Molecular Weight: 683 aa, 77 kDa Observed Molecular Weight: 75-85 kDa GenBank Accession Number: BC136332 Gene Symbol: CKAP2 Gene ID (NCBI): 26586 RRID: AB_2880100 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WWK9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.