Proteintech, 25523-1-AP, CTTNBP2NL Polyclonal antibody

Size: 20ul / 150ul The CTTNBP2NL (25523-1-AP) by Proteintech is a Polyclonal antibody targeting CTTNBP2NL in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 25523-1-AP targets CTTNBP2NL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, MDA-MB-453s cells, mouse skin tissue, MCF-7 cells Positive IP detected in: MCF-7 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22137 Product name: Recombinant human CTTNBP2NL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 271-479 aa of BC016029 Sequence: KKIVKDLEASHQHSSPNEQLKKPVTVSKGTATEPLMLMSVFCQTESFPAERTHGSNIAKMTNTGLPGPATPAYSYAKTNGHCDPEIQTTRELTAGNNVENQVPPREKSVALAQEKPVENGGCPVGIETPVPMPSPLSSSGSSLSPSSTASSSLTSSPCSSPVLTKRLLGSSASSPGYQSSYQVGINQRFHAARHKFQSQADQDQQASGL Predict reactive species Full Name: CTTNBP2 N-terminal like Calculated Molecular Weight: 639 aa, 70 kDa Observed Molecular Weight: 70-75 kDa GenBank Accession Number: BC016029 Gene Symbol: CTTNBP2NL Gene ID (NCBI): 55917 RRID: AB_2880117 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P2B4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.