Proteintech, 25621-1-AP, NDUFAF3 Polyclonal antibody

Size: 20ul / 150ul The NDUFAF3 (25621-1-AP) by Proteintech is a Polyclonal antibody targeting NDUFAF3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 25621-1-AP targets NDUFAF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information NDUFAF3 has been assigned to be an assembly factor of the Q-module because it physically associates with Q-module subassemblies during complex I biogenesis (PMID: 27986404). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22514 Product name: Recombinant human NDUFAF3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-184 aa of BC002873 Sequence: MATALALRSLYRARPSLRCPPVELPWAPRRGHRLSPADDELYQRTRISLLQREAAQAMYIDSYNSRGFMINGNRVLGPCALLPHSVVQWNVGSHQDITEDSFSLFWLLEPRIEIVVVGTGDRTERLQSQVLQAMRQRGIAVEVQDTPNACATFNFLCHEGRVTGAALIPPPGGTSLTSLGQAAQ Predict reactive species Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 Calculated Molecular Weight: 184 aa, 20 kDa Observed Molecular Weight: 17 kDa GenBank Accession Number: BC002873 Gene Symbol: NDUFAF3 Gene ID (NCBI): 25915 RRID: AB_3669488 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BU61 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.