Proteintech, 25625-1-AP, CHCHD3/MIC19 Polyclonal antibody

Size: 20ul / 150ul The CHCHD3/MIC19 (25625-1-AP) by Proteintech is a Polyclonal antibody targeting CHCHD3/MIC19 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 25625-1-AP targets CHCHD3/MIC19 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, human placenta tissue, HeLa cells, Jurkat cells, NIH/3T3 cells, NIH/3T3 cells., mouse liver tissue, rat liver tissue Positive IP detected in: HeLa cells Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:4000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22579 Product name: Recombinant human CHCHD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-227 aa of BC014839 Sequence: MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG Predict reactive species Full Name: coiled-coil-helix-coiled-coil-helix domain containing 3 Calculated Molecular Weight: 227 aa, 26 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: BC014839 Gene Symbol: CHCHD3 Gene ID (NCBI): 54927 RRID: AB_2687533 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NX63 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.