Proteintech, 25667-1-AP, MARVELD3 Polyclonal antibody

Size: 20ul / 150ul The MARVELD3 (25667-1-AP) by Proteintech is a Polyclonal antibody targeting MARVELD3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 25667-1-AP targets MARVELD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Caco-2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MARVELD3 (MARVEL domain-containing protein 3), a four-span transmembrane protein of about 40 kDa, is a tight junction-associated transmembrane protein of the occludin family (PMID: 20028514). MARVELD3 functions as a determinant of epithelial paracellular permeability properties. It has been reported that MARVELD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behavior and survival (PMID: 24567356). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22492 Product name: Recombinant human MARVELD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC013376 Sequence: MEDPSGAREPRARPRERDPGRRPHPDQGRTHDRPRDRPGDPRRKRSSDGNRRRDGDRDPKRDQERDGNRDRNRDRERERERERDPDRGPRRDTHRDAGPRAGEHGVWEKPRQSRTRDGARGLTWDAAAPPGPAPWEAPEPPQPQRKGDPGRRRPESEPPSERYLPSTPRPGREEVEYYQSEAEGLLECH Predict reactive species Full Name: MARVEL domain containing 3 Calculated Molecular Weight: 401 aa, 45 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC013376 Gene Symbol: MARVELD3 Gene ID (NCBI): 91862 RRID: AB_2880184 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96A59 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.