Proteintech, 25710-1-AP, UHRF2 Polyclonal antibody

Size: 20ul / 150ul The UHRF2 (25710-1-AP) by Proteintech is a Polyclonal antibody targeting UHRF2 in WB, IF/ICC, IP, ELISA applications with reactivity to human samples 25710-1-AP targets UHRF2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells, HeLa cells, Jurkat cells, PC-3 cells Positive IP detected in: Jurkat cells Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Ubiquitin-Like with PHD and ring finger domains 2 (UHRF2), a member that belongs to the family of UHRF, contains five recognizable functional domains, namely the ubiquitin-like domain (UBL) domain, tandem-Tudor domain (TTD), plant homeodomain (PHD), SET and RING associated (SRA) domain, and really interesting new gene (RING) finger domain. Due to the complex structure, UHRF2 possesses multiple functions in diverse cellular processes. As a ubiquitin E3 ligase, UHRF2 could ubiquitinate PCNP, a nuclear protein that contains two remarkable PEST sequences which are rich in proline (P), glutamic acid (E), serine (S), and threonine (T). It has been also reported that UHRF2 could serve as a vital cell cycle regulator by interacting with multiple cyclins, CDKs, p53, pRB and PCNA. UHRF2 has been revealed to possess epigenetic regulation function and is capable of maintaining 5mC levels in certain genomic loci in brain and stabilizes TIP60 to regulate H3K9ac and H3K14ac through RING finger domain. Moreover, UHRF2 could promote DNA damage repair by reducing the level of p21 mediated by RING finger domain. Recently, emerging evidence indicated that UHRF2 was involved in the tumorigenesis and progression of several human cancers, such as esophageal squamous cell carcinoma, lung cancer and colorectal cancer. UHRF2 has 2 isoforms with the molecular mass of 56 and 90 kDa. (PMID: 34400880) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22519 Product name: Recombinant human UHRF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 77-117 aa of BC028397 Sequence: PDHLPGTSTQIEAKPCSNSPPKVKKAPRVGPSNQPSTSARA Predict reactive species Full Name: ubiquitin-like with PHD and ring finger domains 2 Calculated Molecular Weight: 90 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC028397 Gene Symbol: UHRF2 Gene ID (NCBI): 115426 RRID: AB_2935463 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96PU4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.