Proteintech, 25856-1-AP, TUBGCP2 Polyclonal antibody

Size: 20ul / 150ul The TUBGCP2 (25856-1-AP) by Proteintech is a Polyclonal antibody targeting TUBGCP2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 25856-1-AP targets TUBGCP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, U2OS cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TUBGCP2 (tubulin-gamma-complex-associated protein 2) s TUBGCP2 is a core component of the g-TuRC nucleation complex (PMID: 3345861). Pathogenic variants in TUBGCP2 cause an autosomal recessive neurodevelopmental trait consisting of a neuronal migration disord (PMID: 31630790). Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23066 Product name: Recombinant human TUBGCP2 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 553-902 aa of BC093770 Sequence: ALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAMAHADPTELALSGLEAFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHSLHSAQWFAGAFTLRQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLGHHTGFLDTCLKDCMLTNPELLKVFSKLMSVCVMFTNCMQKFTQSMKLDGELGGQTLEHSTVLGLPAGAEERARKELARKHLAEHADTVQLVSGFEATINKFDKNFSAHLLDLLARLSIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKATPQVPVLRGPPAPAPRVAVTAQ Predict reactive species Full Name: tubulin, gamma complex associated protein 2 Calculated Molecular Weight: 902 aa, 103 kDa Observed Molecular Weight: 103 kDa GenBank Accession Number: BC093770 Gene Symbol: TUBGCP2 Gene ID (NCBI): 10844 RRID: AB_2880271 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BSJ2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.