Proteintech, 25914-1-AP, NUDT15 Polyclonal antibody

Size: 20ul / 150ul The NUDT15 (25914-1-AP) by Proteintech is a Polyclonal antibody targeting NUDT15 in WB, ELISA applications with reactivity to human samples 25914-1-AP targets NUDT15 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information NUDT15 (nucleoside diphosphate-linked moiety X-type motif 15) is homologues to NUDT1 (MutT homologue 1, MTH1) and thus has previously been referred to as MTH2. NUDT15 is a key enzyme in thiopurine metabolism and tempers the activity of thiopurine drugs by catalyzing the hydrolysis of 6-thio-dGTP and 6-thio-GTP (PMID: 27530327, 32690945). NUDT15 is a 164-amino-acid protein that belongs to the nudix hydrolase enzyme family, whose members are capable of hydrolyzing compounds with the general structure of a nucleoside diphosphate linked to another moiety, X. (PMID: 25108385) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23242 Product name: Recombinant human NUDT15 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC133015 Sequence: MTASAQPRGRRPGVGVGVVVTSCKHPRCVLLGKRKGSVGAGSFQLPGGHLEFGETWEECAQRETWEEAALHLKNVHFASVVNSFIEKENYHYVTILMKGEVDVTHDSEPKNVEPEKNESWEWVPWEELPPLDQLFWGLRCLKEQGYDPFKEDLNHLVGYKGNHL Predict reactive species Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 15 Observed Molecular Weight: 19 kDa GenBank Accession Number: BC133015 Gene Symbol: NUDT15 Gene ID (NCBI): 55270 RRID: AB_2880294 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NV35 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.