Proteintech, 25941-1-AP, GPA33 Polyclonal antibody

Size: 20ul / 150ul The GPA33 (25941-1-AP) by Proteintech is a Polyclonal antibody targeting GPA33 in WB, ELISA applications with reactivity to human, mouse samples 25941-1-AP targets GPA33 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: COLO 320 cells, HT-29 cells, mouse colon tissue, mouse small intestine tissue, rat colon tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Glycoprotein A33 (GPA33) is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 9862345; 9012807). It contains an extracellular region of 213 amino acids, a transmembrane domain, and a highly polar intracellular tail containing four consecutive cysteine residues (PMID: 9012807). GPA33 is specifically expressed on the basolateral surfaces of intestinal epithelial cells of all lineages and is found in 95% of primary and metastatic colorectal cancers (PMID: 10960348; 10950144; 21541536). GPA33 may have a role in cell adhesion and has been implicated in immune dysregulation (PMID: 9012807; 33728639). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12655 Product name: Recombinant human GPA33 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 26-319 aa of BC074830 Sequence: TPQDVLRASQGKSVTLPCTYHTSTSSREGLIQWDKLLLTHTERVVIWPFSNKNYIHGELYKNRVSISNNAEQSDASITIDQLTMADNGTYECSVSLMSDLEGNTKSRVRLLVLVPPSKPECGIEGETIIGNNIQLTCQSKEGSPTPQYSWKRYNILNQEQPLAQPASGQPVSLKNISTDTSGYYICTSSNEEGTQFCNITVAVRSPSMNVALYVGIAVGVVAALIIIGIIIYCCCCRGKDDNTEDKEDARPNREAYEEPPEQLRELSREREEEDDYRQEEQRSTGRESPDHLDQ Predict reactive species Full Name: glycoprotein A33 (transmembrane) Calculated Molecular Weight: 319 aa, 36 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC074830 Gene Symbol: GPA33 Gene ID (NCBI): 10223 RRID: AB_3669509 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99795 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.