Proteintech, 26003-1-AP, NDUFAF4 Polyclonal antibody

Size: 20ul / 150ul The NDUFAF4 (26003-1-AP) by Proteintech is a Polyclonal antibody targeting NDUFAF4 in WB, IHC, ELISA applications with reactivity to human samples 26003-1-AP targets NDUFAF4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, MDA-MB-453s cells, K-562 cells Positive IHC detected in: human liver tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information NDUFAF4 is a protein that in humans is encoded by the C6ORF66 gene. NDUFAF4 is an assembly factor of complex I. Mutation of this gene has been associated with congenital disorders of the mitochondrial respiratory chain (PMID: 18179882). NDUFAF4 was previously shown to promote breast cancer cell invasiveness. NDUFAF4 is a 20.2 kDa mitochondrial protein. Catalog#26003-1-AP specifically recognises the 20 kDa protein. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23365 Product name: Recombinant human NDUFAF4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC039464 Sequence: MGALVIRGIRNFNLENRAEREISKMKPSVAPRHPSTNSLLREQISLYPEVKGEIARKDEKLLSFLKDVYVDSKDPVSSLQVKAAETCQEPKEFRLPKDHHFDMINIKSIPKGKISIVEALTLLNNHKLFPETWTAEKIMQEYQLEQKDVNSLLKYFVTFEVEIFPPEDKKAIRSK Predict reactive species Full Name: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4 Observed Molecular Weight: 21 kDa GenBank Accession Number: BC039464 Gene Symbol: NDUFAF4 Gene ID (NCBI): 29078 RRID: AB_2880329 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P032 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.