Proteintech, 26011-1-AP, RSF1 Polyclonal antibody

Size: 20ul / 150ul The RSF1 (26011-1-AP) by Proteintech is a Polyclonal antibody targeting RSF1 in WB, ELISA applications with reactivity to human samples 26011-1-AP targets RSF1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells, HeLa cells, HEK-293 cells, U-251 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information RSF1, remodelling and spacing factor 1, is an important interphase centromere protein and is overexpressed in many types of cancers and correlated with poor overall survival. RSF1 has functions mainly in maintaining chromosome stability, facilitating DNA repair, maintaining the protein homeostasis of RSF1 and suppressing the transcription of some oncogenes when RSF1 protein is expressed at an optimal level; however, RSF1 overexpression facilitates drug resistance and cell cycle checkpoint inhibition to prompt cancer proliferation and survival. The RSF1 expression level and gene background are crucial for RSF1 functions, which may explain why RSF1 has different functions in different cancer types. The calculated molecular weight of RSF1 is 163 kDa, but 26011-1-AP can detect a band around 250 kDa, we validated the specificity by knock down. (PMID: 34147108) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22456 Product name: Recombinant human RSF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1093-1441 aa of BC153210 Sequence: DLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKKKYSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSESEESYLSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEEEGKPSRKRLHRIETDEEESCDNAHGDANQPARDSQPRVLPSEQESTKKPYRIESDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPTEKSQTPKDNSTASASLASNGTSGGQEAGAPEEEEDELLRVTDLVDYVCNSEQL Predict reactive species Full Name: remodeling and spacing factor 1 Calculated Molecular Weight: 1441 aa, 164 kDa Observed Molecular Weight: 250 kDa GenBank Accession Number: BC153210 Gene Symbol: RSF1 Gene ID (NCBI): 51773 RRID: AB_3085834 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96T23 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.