Proteintech, 26013-1-AP, CEP83 Polyclonal antibody

Size: 20ul / 150ul The CEP83 (26013-1-AP) by Proteintech is a Polyclonal antibody targeting CEP83 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 26013-1-AP targets CEP83 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: hTERT-RPE1 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CEP83 (centrosomal protein, 83 kDa), also known as CCDC41 (coiled-coil domain containing 41), is required for ciliary vesicle docking to the mother centriole. It is a key component of the centriolar distal appendages. CCDC41 may collaborate with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium during the initiation of primary cilium assembly (PMID: 23530209). Alternative splicing results in transcript variants encoding two isoforms with calculated molecular weights of 83 kDa and 68 kDa, respectively. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag22826 Product name: Recombinant human CCDC41 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 226-568 aa of BC125087 Sequence: MQATVRSLEAEKQSANLRAERLEKELQSSSEQNTFLINKLHKAEREINTLSSKVKELKHSNKLEITDIKLETARAKSELERERNKIQSELDGLQSDNEILKAAVEHHKVLLVEKDRELIRKVQAAKEEGYQKLVVLQDEKLELENRLADLEKMKVEHDVWRQSEKDQYEEKLRASQMAEEITRKELQSVRLKLQQQIVTIENAEKEKNENSDLKQQISSLQIQVTSLAQSENDLLNSNQMLKEMVERLKQECRNFRSQAEKAQLEAEKTLEEKQIQWLEEKHKLHERITDREEKYNQAKEKLQRAAIAQKKLEQDLELGCPSVTDTYRESVFPPPPLKRDLLK Predict reactive species Full Name: coiled-coil domain containing 41 Calculated Molecular Weight: 693 aa, 82 kDa Observed Molecular Weight: 83 kDa, 68 kDa GenBank Accession Number: BC125087 Gene Symbol: CEP83 Gene ID (NCBI): 51134 RRID: AB_2880334 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y592 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.