Proteintech, 26047-1-AP, IGSF2/CD101 Polyclonal antibody

Size: 20ul / 150ul The IGSF2/CD101 (26047-1-AP) by Proteintech is a Polyclonal antibody targeting IGSF2/CD101 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 26047-1-AP targets IGSF2/CD101 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HCT 116 cells, RAW 264.7 cells, Jurkat cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2. This antibody specifically recognises the 115 kDa protein. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23297 Product name: Recombinant human IGSF2 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 260-603 aa of BC136484 Sequence: ETWMFITKKQTDQTTLRIQPAVKDFQVNITADSLFAEGKPLELVCLVVSSGRDPQLQGIWFFNGTEIAHIDAGGVLGLKNDYKERASQGELQVSKLGPKAFSLKIFSLGPEDEGAYRCVVAEVMKTRTGSWQVLQRKQSPDSHVHLRKPAARSVVVSTKNKQQVVWEGETLAFLCKAGGAESPLSVSWWHIPRDQTQPEFVAGMGQDGIVQLGASYGVPSYHGNTRLEKMDWATFQLEITFTAITDSGTYECRVSEKSRNQARDLSWTQKISVTVKSLESSLQVSLMSRQPQVMLTNTFDLSCVVRAGYSDLKVPLTVTWQFQPASSHIFHQLIRITHNGTIEW Predict reactive species Full Name: immunoglobulin superfamily, member 2 Observed Molecular Weight: 115 kDa GenBank Accession Number: BC136484 Gene Symbol: IGSF2 Gene ID (NCBI): 9398 RRID: AB_2880350 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q93033 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.