Proteintech, 26072-1-AP, PARP10 Polyclonal antibody

Size: 20ul / 150ul The PARP10 (26072-1-AP) by Proteintech is a Polyclonal antibody targeting PARP10 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 26072-1-AP targets PARP10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HL-60 cells, MCF-7 cells Positive IP detected in: Jurkat cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PARP10 (Poly(ADP-ribose) polymerase family member 10) is a mono-ADP-ribosyltransferase that belongs to the PARP family. It regulates a variety of cellular functions, including gene transcription, DNA repair, and cell cycle control, by catalyzing ADP-ribosylation reactions. PARP10 is distributed in both the nucleus and cytoplasm, participating in the cellular response to DNA damage. In particular, its expression level significantly increases in response to UV-induced DNA damage. Additionally, PARP10 plays a crucial role in the antiviral response, being highly upregulated by interferon (IFN) to inhibit the replication of certain viruses and block protein translation during viral replication. PARP10 is also involved in immune regulation, interacting with NEMO to block the NF-κB signaling pathway and the production of pro-inflammatory cytokines. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23457 Product name: Recombinant human PARP10 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 673-1025 aa of BC136542 Sequence: QEEAAALRQALTLSLLEQPPLEAEEPPDGGTDGKAQLVVHSAFEQDVEELDRALRAALEVHVQEETVGPWRRTLPAELRARLERCHGVSVALRGDCTILRGFGAHPARAARHLVALLAGPWDQSLAFPLAASGPTLAGQTLKGPWNNLERLAENTGEFQEVVRAFYDTLDAARSSIRVVRVERVSHPLLQQQYELYRERLLQRCERRPVEQVLYHGTTAPAVPDICAHGFNRSFCGRNATVYGKGVYFARRASLSVQDRYSPPNADGHKAVFVARVLTGDYGQGRRGLRAPPLRGPGHVLLRYDSAVDCICQPSIFVIFHDTQALPTHLITCEHVPRASPDDPSGLPGRSPDT Predict reactive species Full Name: poly (ADP-ribose) polymerase family, member 10 Observed Molecular Weight: 130-140 kDa GenBank Accession Number: BC136542 Gene Symbol: PARP10 Gene ID (NCBI): 84875 RRID: AB_2880362 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q53GL7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.