Product Description
Size: 20ul / 150ul
The NBCe2 (26150-1-AP) by Proteintech is a Polyclonal antibody targeting NBCe2 in IHC, IF/ICC, ELISA applications with reactivity to Human, Mouse samples
26150-1-AP targets NBCe2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Applications
Positive IHC detected in: human liver cancer tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: HepG2 cells
Recommended dilution
Immunohistochemistry (IHC): IHC : 1:50-1:500
Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
Background Information
NBCe2, also named as SLC4A5 and NBC4, belongs to the anion exchanger (TC 2.A.31) family. NBCe2 mediates electrogenic Na+:HCO3 − transport, and can operate in both a 1:2, and 1:3 manner, but the transport stoichiometry as well as directionality have not been defined for the renal NBCe2. The expression of NBCe2 has been reported in nearly all kidney nephron segments and CDs, indicating that some species-specificity might be present (PMID: 32547422). NBCe2 has 8 isoforms with the molecular mass of 108-127 kDa.
Specification
Tested Reactivity: Human, Mouse
Cited Reactivity: human, mouse
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Immunogen: CatNo: Ag23558 Product name: Recombinant human SLC4A5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 953-1019 aa of BC109221 Sequence: NILPEKEKKETDKKRKRKKGAHEDCDEEPQFPPPSVIKIPMESVQSDPQNGIHCIARKRSSSWSYSL Predict reactive species
Full Name: solute carrier family 4, sodium bicarbonate cotransporter, member 5
Observed Molecular Weight: 108-127 kDa
GenBank Accession Number: BC109221
Gene Symbol: SLC4A5
Gene ID (NCBI): 57835
RRID: AB_2918099
Conjugate: Unconjugated
Form: Liquid
Purification Method: Antigen affinity purification
UNIPROT ID: Q9BY07
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
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