Proteintech, 26225-1-AP, PATZ1 Polyclonal antibody

Size: 20ul / 150ul The PATZ1 (26225-1-AP) by Proteintech is a Polyclonal antibody targeting PATZ1 in WB, ELISA applications with reactivity to human, mouse samples 26225-1-AP targets PATZ1 in WB, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse spleen tissue, Raji cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information PATZ1 (POZ/BTB and AT-hook containing Zinc finger protein 1) is an important multifunctional transcriptional regulator. Its protein structure is distinctive, containing a POZ/BTB domain (which mediates protein-protein interactions and transcriptional repression) and an AT-hook motif (which binds to AT-rich DNA sequences), as well as multiple C2H2-type zinc finger domains (responsible for sequence-specific DNA binding). PATZ1 plays a "double-edged sword" role in regulating gene expression, capable of either activating or repressing target gene transcription depending on the co-regulators it interacts with and the cellular context. It plays a critical role in embryonic development and in the maintenance of stem cell pluripotency and differentiation. In recent years, its dual role in cancer has garnered significant attention. In various tumors (such as glioma, sarcoma, and colorectal cancer), PATZ1 can function as either an oncogene or a tumor suppressor depending on the context, participating in tumorigenesis and progression by influencing pathways such as the cell cycle, apoptosis, and the DNA damage response. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23912 Product name: Recombinant human PATZ1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC021091 Sequence: MERVNDASCGPSGCYTYQVSRHSTEMLHNLNQQRKNGGRFCDVLLRVGDESFPAHRAVLAACSEYFESVFSAQLGDGGAADGGPADVGGATAAPGGGAGGSRELEMHTISSKVFGDILDFAYTSRIVVRLESFPELMTAAKFLLMRSVIEICQEVIKQSNVQILVPPARADIMLFRPPGTSDLGFPLDMTNGAALAANSNGIAGSMQPEEEAARAAGAAIAGQASLPVLPGVDRLPMVAGPLSPQLLTSPFPSVASSAPPLTGKRGRGRPRKANLLDSMFGSPGGLREAGILPCGLCGKVFTDANRLRQHEAQHGVTSLQLGYIDLPPPRLGENGLPISEDPDGPRKRSR Predict reactive species Full Name: POZ (BTB) and AT hook containing zinc finger 1 Observed Molecular Weight: 70-74 kDa GenBank Accession Number: BC021091 Gene Symbol: PATZ1 Gene ID (NCBI): 23598 RRID: AB_2880432 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9HBE1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.