Proteintech, 26446-1-AP, NPRL3 Polyclonal antibody

Size: 20ul / 150ul The NPRL3 (26446-1-AP) by Proteintech is a Polyclonal antibody targeting NPRL3 in WB, ELISA applications with reactivity to human samples 26446-1-AP targets NPRL3 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: K-562 cells, LNCaP cells, THP-1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information NPRL3 (Nitrogen Permease Regulator Like 3) is a highly conserved tumor suppressor protein. As a core component of the GATOR1 complex, it serves as a key node in the amino acid sensing pathway and negatively regulates the mTORC1 signaling pathway, thereby controlling cell growth, proliferation, and autophagy. Loss of its function leads to hyperactivation of mTORC1 signaling. In neuroscience, loss-of-function mutations in NPRL3 are an important genetic cause of familial focal epilepsy, such as focal epilepsy with variable foci. In oncology, it is frequently inactivated through epigenetic silencing in various cancers, making it a significant tumor suppressor and a potential biomarker. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag24045 Product name: Recombinant human C16orf35 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-390 aa of BC004185 Sequence: MADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAASSLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQDADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHDLPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEEPRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQRMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQLLMLFDKFRSVLVVTTHEDPVIAVFQALLP Predict reactive species Full Name: chromosome 16 open reading frame 35 Calculated Molecular Weight: 64 kDa Observed Molecular Weight: 63 kDa GenBank Accession Number: BC004185 Gene Symbol: C16orf35 Gene ID (NCBI): 8131 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q12980 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.