Proteintech, 26533-1-AP, Ephrin B2 Polyclonal antibody

Size: 20ul / 150ul The Ephrin B2 (26533-1-AP) by Proteintech is a Polyclonal antibody targeting Ephrin B2 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 26533-1-AP targets Ephrin B2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse lung tissue, mouse placenta tissue, mouse spleen tissue Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Ephrin B2 (EFNB2) is a transmembrane ligand for the Ephrin B class (EPHB) of receptor tyrosine kinases. EPHB receptor(EPHB1-EPHB4, EPH6) are located at the member of one cell and activated by binding EFNB protein located on another cell, resulting in a bidirectional Ephrin-EphR signaling (PMID: 26790531). Ephrin-B are involved in vasculogenesis, neural development and synaptic plasticity, and in pathological processes such as cancer or neurodegeneration (PMID:29508392). The calculated molecular weight of EFNB2 is 37 kDa, and there is also detected about 50 kDa band in the article (PMID: 27650867, PMID: 23492730). Specification Tested Reactivity: human, mouse Cited Reactivity: hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag24865 Product name: Recombinant human EFNB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 168-235 aa of BC074856 Sequence: ASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAGIASGC Predict reactive species Full Name: ephrin-B2 Calculated Molecular Weight: 333 aa, 37 kDa Observed Molecular Weight: 47-50 kDa GenBank Accession Number: BC074856 Gene Symbol: EFNB2 Gene ID (NCBI): 1948 RRID: AB_3085878 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P52799 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.