Proteintech, 26709-1-AP, BSAP,PAX5 Polyclonal antibody

Size: 20ul / 150ul The BSAP,PAX5 (26709-1-AP) by Proteintech is a Polyclonal antibody targeting BSAP,PAX5 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 26709-1-AP targets BSAP,PAX5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Daudi cells, Raji cells, Ramos cells Positive IP detected in: Ramos cells Positive IHC detected in: human tonsillitis tissue, human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:150-1:600 Background Information PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25147 Product name: Recombinant human BSAP,PAX5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 362-391 aa of BC156927 Sequence: NPGLLGSPYYYSAAARGAAPPAAATAYDRH Predict reactive species Full Name: paired box 5 Calculated Molecular Weight: 391 aa, 42 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC156927 Gene Symbol: PAX5 Gene ID (NCBI): 5079 RRID: AB_2880608 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q02548 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.