Proteintech, 26755-1-AP, Cyclin D3 Polyclonal antibody

Size: 20ul / 150ul The Cyclin D3 (26755-1-AP) by Proteintech is a Polyclonal antibody targeting Cyclin D3 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, rat samples 26755-1-AP targets Cyclin D3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: C6 cells, K-562 cells, Jurkat cells, HeLa cells Positive IP detected in: K-562 cells Positive IHC detected in: human pancreas tissue, rat kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse, rat, sheep Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25151 Product name: Recombinant human Cyclin D3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 250-292 aa of BC011616 Sequence: ALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL Predict reactive species Full Name: cyclin D3 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC011616 Gene Symbol: Cyclin D3 Gene ID (NCBI): 896 ENSEMBL Gene ID: ENSG00000112576 RRID: AB_2756524 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P30281 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.