Proteintech, 26787-1-AP, YTHDF1 Polyclonal antibody

Size: 20ul / 150ul The YTHDF1 (26787-1-AP) by Proteintech is a Polyclonal antibody targeting YTHDF1 in WB, IP, ELISA applications with reactivity to human samples 26787-1-AP targets YTHDF1 in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells Positive IP detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information YTHDF1, also named YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at the internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing, and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoforms and the calculated molecular weight of isoforms are 61 kDa and 21 kDa. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25347 Product name: Recombinant human YTHDF1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 284-325 aa of BC050284 Sequence: PVPQQAPSPQAAPQPQQVAQPLPAQPPALAQPQYQSPQQPPQ Predict reactive species Full Name: YTH domain family, member 1 Calculated Molecular Weight: 559 aa, 61 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC050284 Gene Symbol: YTHDF1 Gene ID (NCBI): 54915 RRID: AB_2880635 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BYJ9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.