Proteintech, 26870-1-AP, TIAM2 Polyclonal antibody

Size: 20ul / 150ul The TIAM2 (26870-1-AP) by Proteintech is a Polyclonal antibody targeting TIAM2 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 26870-1-AP targets TIAM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: BxPC-3 cells, U2OS cells, mouse testis tissue, rat testis tissue, HEK-293 cells Positive IF/ICC detected in: RAW 264.7 cells Positive FC (Intra) detected in: K-562 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information TIAM2 is a Rac1-specific GEF, which was identified originally as a homologue of TIAM1. TIAM2 plays a critical role in the malignancy development of many human cancers. TIAM2 expression upregulates N-cadherin and vimentin, thus enhancing epithelial-to-mesenchymal transition (EMT) and leading to the enhancement of proliferation and invasion in hepatoma cells. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25469 Product name: Recombinant human TIAM2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1346-1701 aa of BC137275 Sequence: KDLELTVFVFKRAVILVYKENCKLKKKLPSNSRPAHNSTDLDPFKFRWLIPISALQVRLGNPAGTENNSIWELIHTKSEIEGRPETIFQLCCSDSESKTNIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKLASSRSLKVLKNSSSNEWTGETGKGTLLDSDEGSLSSGTQSSGCPTAEGRQDSKSTSPGKYPHPGLADFADNLIKESDILSDEDDDHRQTVKQGSPTKDIEIQFQRLRISEDPDVHPEAEQQPGPESGEGQKGGEQPKLVRGHFCPIKRKANSTKRDRGTLLKAQIRHQSLDSQSENATIDLNSVLEREFSVQSLTSVVSEECFYETESHGKS Predict reactive species Full Name: T-cell lymphoma invasion and metastasis 2 Observed Molecular Weight: 190-200 kDa GenBank Accession Number: BC137275 Gene Symbol: TIAM2 Gene ID (NCBI): 26230 RRID: AB_3085914 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IVF5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.