Proteintech, 26939-1-AP, Cyclin D1 Polyclonal antibody

Size: 20ul / 150ul The Cyclin D1 (26939-1-AP) by Proteintech is a Polyclonal antibody targeting Cyclin D1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 26939-1-AP targets Cyclin D1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: LNCaP cells, C6 cells, MCF-7 cells, NIH/3T3 cells, Neuro-2a cells, mouse lung tissue Positive IHC detected in: human ovary tumor tissue, human oesophagus cancer tissue, human lung cancer tissue, mouse spleen tissue, mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells, NIH/3T3 cells Positive FC (Intra) detected in: SH-SY5Y cells, MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:750-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis. Cyclin D1 may have a molecular weight of 45kDa due to SUMO modification. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, canine, bovine, sheep, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25393 Product name: Recombinant human Cyclin D1 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 193-295 aa of BC000076 Sequence: VKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI Predict reactive species Full Name: cyclin D1 Calculated Molecular Weight: 295 aa, 34 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC000076 Gene Symbol: Cyclin D1 Gene ID (NCBI): 595 RRID: AB_2880691 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P24385 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.