Proteintech, 27026-1-AP, LPIN1 Polyclonal antibody

Size: 20ul / 150ul The LPIN1 (27026-1-AP) by Proteintech is a Polyclonal antibody targeting LPIN1 in WB, IP, IHC, ELISA applications with reactivity to human samples 27026-1-AP targets LPIN1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, LNCaP cells Positive IP detected in: LNCaP cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Specification Tested Reactivity: human Cited Reactivity: human, mouse, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25815 Product name: Recombinant human LPIN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 301-460 aa of BC030537 Sequence: ICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRD Predict reactive species Full Name: lipin 1 Calculated Molecular Weight: 890 aa, 99 kDa Observed Molecular Weight: 110-130 kDa GenBank Accession Number: BC030537 Gene Symbol: LPIN1 Gene ID (NCBI): 23175 RRID: AB_2880727 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14693 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.