Proteintech, 27200-1-AP, C8G Polyclonal antibody

Size: 20ul / 150ul The C8G (27200-1-AP) by Proteintech is a Polyclonal antibody targeting C8G in WB, IHC, ELISA applications with reactivity to human samples 27200-1-AP targets C8G in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human milk, human placenta tissue Positive IHC detected in: human breast cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The gamma chain of complement component 8 (C8G) is one of three different subunits (alpha, beta, and gamma) of the eighth complement component (C8), which is a component of the MAC (membrane attack complex) (PMID:33382892). Although the roles of C8A and C8B are directly associated with MAC formation, C8G is not essential in the assembly and activity of the MAC (PMID:11960635). Moreover, C8G was shown to inhibit neuroinflammation by interfering with the sphingosine-1-phosphate (S1P)-S1PR2 interaction (PMID:34149451). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17949 Product name: Recombinant human C8G protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-202 aa of BC113626 Sequence: MLPPGTATLLTLLLAAGSLGQKPQRPRRPASPISTIQPKANFDAQQFAGTWLLVAVGSACRFLQEQGHRAEATTLHVAPQGTAMAVSTFRKLDGICWQVRQLYGDTGVLGRFLLQARGARGAVNVVVAETDYQSFAVLYLERAGQLSVKLYARSLPVSDSVLSGFEQRVQEAHLTEDQIFYFPKYGFCEAADQFHVLDEVRR Predict reactive species Full Name: complement component 8, gamma polypeptide Calculated Molecular Weight: 202 aa, 22 kDa Observed Molecular Weight: 18-22 kDa GenBank Accession Number: BC113626 Gene Symbol: C8G Gene ID (NCBI): 733 RRID: AB_3085935 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P07360 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.