Proteintech, 27684-1-AP, SF3B1 Polyclonal antibody

Size: 20ul / 150ul The SF3B1 (27684-1-AP) by Proteintech is a Polyclonal antibody targeting SF3B1 in WB, IHC, ELISA applications with reactivity to Human samples 27684-1-AP targets SF3B1 in WB, IHC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: MCF-7 cells, HeLa cells, U2OS cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Specification Tested Reactivity: Human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26689 Product name: Recombinant human SF3B1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-126 aa of NM_012433 Sequence: MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTM Predict reactive species Full Name: splicing factor 3b, subunit 1, 155kDa Calculated Molecular Weight: 146 kDa Observed Molecular Weight: 146-150 kDa GenBank Accession Number: NM_012433 Gene Symbol: SF3B1 Gene ID (NCBI): 23451 RRID: AB_2880946 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.