Proteintech, 27723-1-AP, RRP1B Polyclonal antibody

Size: 20ul / 150ul The RRP1B (27723-1-AP) by Proteintech is a Polyclonal antibody targeting RRP1B in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 27723-1-AP targets RRP1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Ribosomal RNA Processing 1 Homolog B (RRP1B) is a protein that has been implicated in several biological processes, particularly in the context of cancer progression and viral replication. RRP1B was first identified as a metastasis susceptibility gene in breast cancer through its ability to modulate gene expression in a manner that can be used to accurately predict prognosis in breast cancer . It has been shown to interact with the metastasis modifier gene, Sipa1, and modulate elements of metastasis predictive gene expression signatures. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag23405 Product name: Recombinant human RRP1B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 386-706 aa of BC028386 Sequence: CVEEEDSESSLQKRRRKKKKKHHLQPENPGPGGAAPSLEQNRGREPEASGPKALKARVAEPGAEATSSTGEESGSEHPPAVPMHNKRKRPRKKSPRAHREMLESAVLPPEDMSQSGPSGSHPQGPRGSPTGGAQLLKRKRKLGVVPVNGSGLSTPAWPPLQQEGPPTGPAEGANSHTTLPQRRRLQKKKAGPGSLELCGLPSQKTASLKKRKKMRVMSNLVEHNGVLESEAGQPQALGSSGTCSSLKKQKLRAESDFVKFDTPFLPKPLFFRRAKSSTATHPPGPAVQLNKTPSSSKKVTFGLNRNMTAEFKKTDKSILVS Predict reactive species Full Name: ribosomal RNA processing 1 homolog B (S. cerevisiae) Observed Molecular Weight: 84 kDa GenBank Accession Number: BC028386 Gene Symbol: RRP1B Gene ID (NCBI): 23076 RRID: AB_2880953 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q14684 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.