Proteintech, 27737-1-AP, Nexilin Polyclonal antibody

Size: 20ul / 150ul The Nexilin (27737-1-AP) by Proteintech is a Polyclonal antibody targeting Nexilin in WB, IHC, IF-P, ELISA applications with reactivity to Human, mouse, rat samples 27737-1-AP targets Nexilin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information Nexilin, Also known as NEXN, NELIN, is a filamentous actin-binding protein that localizes to focal contacts and may be involved in cell adhesion and migration(PMID: 15823560). Nexilin is abundantly expressed in heart and skeletal muscle and has an essential role in the maintenance of Z line and sarcomere integrity(PMID: 12053183, 19881492). Mutations in Nexilin have been associated with cardiomyopathy dilated type 1CC (CMD1CC) and cardiomyopathy familial hypertrophic type 20 (CMH20)(PMID: 19881492, 20970104). Specification Tested Reactivity: Human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag26938 Product name: Recombinant human NEXN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 109-361 aa of BC114444 Sequence: MEKQRQEEQRKRTEEERKRRIEQDMLEKRKIQRELAKRAEQIEDINNTGTESASEEGDDSLLITVVPVKSYKTSGKMKKNFEDLEKEREEKERIKYEEDKRIRYEEQRPSLKEAKCLSLVMDDEIESEAKKESLSPGKLKLTFEELERQRQENRKKQAEEEARKRLEEEKRAFEEARRQMVNEDEENQDTAKIFKGYRPGKLKLSFEEMERQRREDEKRKAEEEARRRIEEEKKAFAEARRNMVVDDDSPEMY Predict reactive species Full Name: nexilin (F actin binding protein) Calculated Molecular Weight: 675 aa, 81 kDa Observed Molecular Weight: 80-85 kDa GenBank Accession Number: BC114444 Gene Symbol: Nexilin Gene ID (NCBI): 91624 RRID: AB_3085991 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q0ZGT2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.