Proteintech, 27783-1-AP, ANXA10 Polyclonal antibody

Size: 20ul / 150ul The ANXA10 (27783-1-AP) by Proteintech is a Polyclonal antibody targeting ANXA10 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 27783-1-AP targets ANXA10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse stomach tissue, rat stomach tissue Positive IHC detected in: rat stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ANXA10 (annexin A10) is a member of the annexin family, and its biological effects are mediated primarily through the calcium-dependent phospholipid-binding and calcium ion binding. ANXA10 is frequently found to be highly expressed in human oral cancer, and this high expression may promote G1 phase cell cycle progression by activating the ERK/MAPK signalling pathway, which in turn results in the reduced expression of member-dependent kinases of cell cycle proteinsn and proliferation. While ANXA10 plays a diverse role in different tumours, in hepatocellular carcinoma, the overexpression of ANXA10 significantly promotes apoptosis. Conversely, in papillary thyroid cancer, the knockdown of ANXA10 promotes apoptosis by inhibiting the MAPK/ERK signalling pathway through the downregulation of TSG101 (PMID: 36936694). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27094 Product name: Recombinant human ANXA10 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 160-324 aa of BC007320 Sequence: QGTREEGYTDPAMAAQDAMVLWEACQQKTGEHKTMLQMILCNKSYQQLRLVFQEFQNISGQDMVDAINECYDGYFQELLVAIVLCVRDKPAYFAYRLYSAIHDFGFHNKTVIRILIARSEIDLLTIRKRYKERYGKSLFHDIRNFASGHYKKALLAICAGDAEDY Predict reactive species Full Name: annexin A10 Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC007320 Gene Symbol: ANXA10 Gene ID (NCBI): 11199 RRID: AB_3669622 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UJ72 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.