Proteintech, 27808-1-AP, PARG Polyclonal antibody

Size: 20ul / 150ul The PARG (27808-1-AP) by Proteintech is a Polyclonal antibody targeting PARG in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 27808-1-AP targets PARG in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells Positive IP detected in: Jurkat cells, mouse testis tissue Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Poly(ADP-ribose) glycohydrolase (PARG) is an enzyme that reverses the PARylation process by breaking down long chains of PAR polymers on proteins, thus having an opposite function to PARP. It hydrolyzes PAR into ADP-ribose units, terminating signals initiated by PARP enzymes. PARG's function is crucial for maintaining PAR balance within cells, which is essential for DNA repair, transcriptional regulation, and cell death. PARG possesses multiple isoforms with distinct subcellular localizations, and its activity is essential for cellular survival. (PMID: 38580335, PMID: 39546575) Specification Tested Reactivity: human, mouse Cited Reactivity: human, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27137 Product name: Recombinant human PARG protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 183-441 aa of BC052966 Sequence: SNANIDRSPQNDDHSDTDSEENRDNQQFLTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNKLTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDADIEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKAEDRRKEQWETKHQRTERKIPK Predict reactive species Full Name: poly (ADP-ribose) glycohydrolase Calculated Molecular Weight: 111 kDa Observed Molecular Weight: 100-111 kDa GenBank Accession Number: BC052966 Gene Symbol: PARG Gene ID (NCBI): 8505 RRID: AB_2880979 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86W56 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.