Proteintech, 27834-1-AP, FMNL1 Polyclonal antibody

Size: 20ul / 150ul The FMNL1 (27834-1-AP) by Proteintech is a Polyclonal antibody targeting FMNL1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 27834-1-AP targets FMNL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Raji cells, HeLa cells, Jurkat cells Positive IP detected in: Caki-1 Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 27834-1-AP is a rabbit polyclonal antibody recognises the 125-150 kDa protein.(PMID:16494944) Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27312 Product name: Recombinant human FMNL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 357-501 aa of BC111564 Sequence: LYLERLRLTESDKLQVQIQAYLDNIFDVGALLEDTETKNAVLEHMEELQEQVALLTERLRDAENESMAKIAELEKQLSQARKELETLRERFSESTAMGPSRRPPEPEKAPPAAPTRPSALELKVEELEEKGLIRILRGPGDAVSI Predict reactive species Full Name: formin-like 1 Observed Molecular Weight: 125-150 kDa GenBank Accession Number: BC111564 Gene Symbol: FMNL1 Gene ID (NCBI): 752 RRID: AB_2880987 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95466 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.