Proteintech, 27864-1-AP, POLR3C Polyclonal antibody

Size: 20ul / 150ul The POLR3C (27864-1-AP) by Proteintech is a Polyclonal antibody targeting POLR3C in WB, IHC, ELISA applications with reactivity to human, mouse samples 27864-1-AP targets POLR3C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293T cells, NIH/3T3 cells, human placenta tissue Positive IHC detected in: mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Mutations in the POLR3A, POLR3C, POLR3E, and POLR3F subunits are associated with susceptibility to varicella zoster virus-induced encephalitis and pneumonitis (PMID: 34395528). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27311 Product name: Recombinant human POLR3C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 196-397 aa of BC002586 Sequence: IGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYK Predict reactive species Full Name: polymerase (RNA) III (DNA directed) polypeptide C (62kD) Calculated Molecular Weight: 61 kDa Observed Molecular Weight: 58 kDa GenBank Accession Number: BC002586 Gene Symbol: POLR3C Gene ID (NCBI): 10623 RRID: AB_3669626 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BUI4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.