Proteintech, 27883-1-AP, MDM2/HDM2 Polyclonal antibody

Size: 20ul / 150ul The MDM2/HDM2 (27883-1-AP) by Proteintech is a Polyclonal antibody targeting MDM2/HDM2 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 27883-1-AP targets MDM2/HDM2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, A431 cells, A549 cells, HepG2 cells, T-47D cells Positive IF/ICC detected in: MG132 treated U2OS cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information What is the cellular localization of MDM2?MDM2 is predominantly localized in the nucleoplasm.What is the tissue specificity of MDM2?MDM2 is non-specific, expressing ubiquitously in the brain, heart, kidney, skin, and other tissues. Certainisoformsonly express in cancerous cells and not normal tissue.With what other molecules does MDM2 interact?Most notably, the oncogene protein MDM2 regulates transcription factor p53 by binding its transcriptionalactivation domain and by E3-ubiquitin ligase activity. Ribosome proteins rpL5, rpL11, and rpL23 also bind toMDM2, inhibiting E3 ubiquitin ligase activity and promoting the p53 pathway.What competes with MDM2 activity?Nutilin-3 was designed to compete with MDM2 in binding with p53, inducing activation of the p53 pathway.What is the role of ubiquitination in MDM2 activity?While p53 is also affected by other post-translational modifications, its stability is determined by ubiquitinationmediation of MDM2, suppressing p53 transcriptional regulation and leading to the proteosomal degradation ofp53.How is MDM2 associated with cancer?MDM2 inhibits the activity of the tumor-suppressive protein p53, which serves as an important factor inpromotingDNA repair or activating apoptotic pathways. Loss of p53 is associated with tumor developmentand growth.What is the human ortholog of MDM2?Human double-minute type 2 (HDM2)In what cellular circumstances does the p53-MDM2 complex dissociate?Cellular stress or DNA damage result in the phosphorylation and activation of p53. Phosphorylation alsoprevents p53-MDM2 interaction.Where is the gene located?Hdm2is located on human 12q15.Mdm2is located on murine 10 D2.PMIDs:24309898, 29584707, 9840926, 28714398 Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27255 Product name: Recombinant human MDM2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 110-298 aa of NM_001145337.2 Sequence: MVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEIS Predict reactive species Full Name: Mdm2 p53 binding protein homolog (mouse) Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 75-85 kDa, 57-60 kDa, 40-55 kDa GenBank Accession Number: NM_001145337.2 Gene Symbol: MDM2 Gene ID (NCBI): 4193 RRID: AB_2881003 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q00987 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.