Proteintech, 27916-1-AP, IL-13Ra1/CD213a1 Polyclonal antibody

Size: 20ul / 150ul The IL-13Ra1/CD213a1 (27916-1-AP) by Proteintech is a Polyclonal antibody targeting IL-13Ra1/CD213a1 in WB, ELISA applications with reactivity to human, mouse samples 27916-1-AP targets IL-13Ra1/CD213a1 in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HT-29 cells, Jurkat cells, Raji cells, mouse skeletal muscle tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information IL-13 is an immunoregulatory cytokine secreted predominantly by activated T-helper 2 (Th2) cells and performs multiple functions through a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2 (PMID: 12704343). IL-13Rα1 is a 65- to 70-kd glycosylated transmembrane protein (PMID: 10856136; 9723655). IL-13Rα1 is expressed on a wide range of cells including B cells, monocytes, macrophages, basophils, mast cells, and endothelial cells (PMID: 10856136; 31866859). IL-13 binding to the IL-4Rα/IL-13Rα1 complex leads to activation of JAK1, JAK2, and TYK2 and subsequent phosphorylation of STAT6 and STAT3 (PMID: 31866859). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25542 Product name: Recombinant human IL-13RA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-277 aa of BC009960 Sequence: MEWPARLCGLWALLLCAGGGGGGGGAAPTETQPPVTNLSVSVENLCTVIWTWNPPEGASSNCSLWYFSHFGDKQDKKIAPETRRSIEVPLNERICLQVGSQCSTNESEKPSILVEKCISPPEGDPESAVTELQCIWHNLSYMKCSWLPGRNTSPDTNYTLYYWHRSLEKIHQCENIFREGQYFGCSFDLTKVKDSSFEQHSVQIMVKDNAGKIKPSFNIVPLTSRVKPDPPHIKNLSFHNDDLYVQWENPQNFISRCLFYEVEVNNSQTETHNVFYV Predict reactive species Full Name: interleukin 13 receptor, alpha 1 Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC009960 Gene Symbol: IL-13Rα1 Gene ID (NCBI): 3597 RRID: AB_3086010 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P78552 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.