Proteintech, 27949-1-AP, CD19 Polyclonal antibody

Size: 20ul / 150ul The CD19 (27949-1-AP) by Proteintech is a Polyclonal antibody targeting CD19 in WB, IHC, IF/ICC, IF-Fro, ELISA applications with reactivity to human, mouse, rat samples 27949-1-AP targets CD19 in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Daudi cells, Raji cells, Ramos cells Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue, mouse colon tissue, rat colon tissue, rat thymus tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-Fro detected in: mouse spleen tissue Positive IF/ICC detected in: Raji cells Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27656 Product name: Recombinant human CD19 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 456-556 aa of BC006338 Sequence: SNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR Predict reactive species Full Name: CD19 molecule Calculated Molecular Weight: 556 aa, 61 kDa Observed Molecular Weight: 95 kDa GenBank Accession Number: BC006338 Gene Symbol: CD19 Gene ID (NCBI): 930 ENSEMBL Gene ID: ENSG00000177455 RRID: AB_2881019 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P15391 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.