Proteintech, 27958-1-AP, DROSHA Polyclonal antibody

Size: 20ul / 150ul The DROSHA (27958-1-AP) by Proteintech is a Polyclonal antibody targeting DROSHA in WB, IF/ICC, ELISA applications with reactivity to human samples 27958-1-AP targets DROSHA in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, HEK-293 cells, Hela cells Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27533 Product name: Recombinant human DROSHA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 353-550 aa of NM_001100412 Sequence: MHRSPSREKKRARWEEEKDRWSDNQSSGKDKNYTSIKEKEPEETMPDKNEEEEEELLKPVWIRCTHSENYYSSDPMDQVGDSTVVGTSRLRDLYDKFEEELGSRQEKAKAARPPWEPPKTKLDEDLESSSESECESDEDSTCSSSSDSEVFDVIAEIKRKKAHPDRLHDELWYNDPGQMNDGPLCKCSAKARRTGIRHS Predict reactive species Full Name: ribonuclease type III, nuclear Calculated Molecular Weight: 159 aa Observed Molecular Weight: 150 kDa GenBank Accession Number: NM_001100412 Gene Symbol: DROSHA Gene ID (NCBI): 29102 RRID: AB_2918137 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NRR4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.