Proteintech, 27965-1-AP, DHFRL1 Polyclonal antibody

Size: 20ul / 150ul The DHFRL1 (27965-1-AP) by Proteintech is a Polyclonal antibody targeting DHFRL1 in WB, IHC, ELISA applications with reactivity to Human, mouse samples 27965-1-AP targets DHFRL1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, Jurkat cells Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information DHFRL1, also known as DHFR2, is a key enzyme in folate metabolism. DHFRL1 is a dihydrofolate reductase, that catalyzes the NADPH-dependent reduction of dihydrofolate to the biologically active form, tetrahydrofolate (PMID: 21876184). Specification Tested Reactivity: Human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10537 Product name: Recombinant human DHFRL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC063379 Sequence: MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGILSDVQEGKHIKYKFEVCEKDD Predict reactive species Full Name: dihydrofolate reductase-like 1 Calculated Molecular Weight: 187 aa, 22 kDa Observed Molecular Weight: 25 kDa GenBank Accession Number: BC063379 Gene Symbol: DHFRL1 Gene ID (NCBI): 200895 RRID: AB_3086015 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86XF0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.