Proteintech, 28022-1-AP, NPTN Polyclonal antibody

Size: 20ul / 150ul The NPTN (28022-1-AP) by Proteintech is a Polyclonal antibody targeting NPTN in WB, ELISA applications with reactivity to human, rat samples 28022-1-AP targets NPTN in WB, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: C6 cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Background Information Neuroplastin (Nptn) is a member of the Ig superfamily and is expressed in two isoforms, Np55 and Np65. Np65 contains three Ig domains (Ig1, Ig2, Ig3). Np55 contains only two (Ig2, Ig3). Np65 regulates synaptic transmission but the function of Np55 is unknown. Expression of the 65 kD neuroplastin (Np65) isoform derived by alternative splicing from the Nptn gene is restricted to brain neurons, whereas the 55 kD isoform (Np55) is present in most cell types but not in glia. (PMID: 33844052, PMID: 33299977, PMID: 27503893,PMID: 27581460) Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag27752 Product name: Recombinant human NPTN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 185-317 aa of BC030773 Sequence: TKNGVELSATRKNASNMEYRINKPRAEDSGEYHCVYHFVSAPKANATIEVKAAPDITGHKRSENKNEGQDATMYCKSVGYPHPDWIWRKKENGMPMDIVNTSGRFFIINKENYTELNIVNLQITEDPGEYECN Predict reactive species Full Name: neuroplastin Calculated Molecular Weight: 282 aa, 31 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC030773 Gene Symbol: NPTN Gene ID (NCBI): 27020 RRID: AB_3086022 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y639 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.