Proteintech, 28162-1-AP, RASEF Polyclonal antibody

Size: 20ul / 150ul The RASEF (28162-1-AP) by Proteintech is a Polyclonal antibody targeting RASEF in WB, IHC, ELISA applications with reactivity to human, rat samples 28162-1-AP targets RASEF in WB, IHC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: LNCaP cells, PC-12 cells Positive IHC detected in: human stomach tissue, human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:3000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information RASEF, or RAS and EF-hand domain-containing protein, is a member of the Rab GTPase protein family. RASEF is a protein with significant implications in lung cancer, potentially serving as both a prognostic biomarker and a therapeutic target. Its role in cancer cell growth and its interaction with ERK1/2 signaling make it a promising candidate for further research and development of new molecular therapies in lung cancer treatment. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag28049 Product name: Recombinant human RASEF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC023566 Sequence: MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGD Predict reactive species Full Name: RAS and EF-hand domain containing Calculated Molecular Weight: 740 aa, 83 kDa Observed Molecular Weight: 90-100 kDa GenBank Accession Number: BC023566 Gene Symbol: RASEF Gene ID (NCBI): 158158 RRID: AB_2881079 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IZ41 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.