Proteintech, 28194-1-AP, FES Polyclonal antibody

Size: 20ul / 150ul The FES (28194-1-AP) by Proteintech is a Polyclonal antibody targeting FES in WB, IHC, ELISA applications with reactivity to Human samples 28194-1-AP targets FES in WB, IHC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HL-60 cells, THP-1 cells, U-937 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Feline sarcoma oncogene protein (FES) is a member of the FES/FES-related (FER) subfamily of non-receptor protein tyrosine kinases. FES was originally identified a retroviral oncogene in avian and feline retroviruses. Articles reported that FES plays a role in growth and differentiation of myeloid hematopoietic cells, vascular endothelial cells and neurons. And it also involved in the regulation of cytoskeletal rearrangement (PMID:15929003). Addition, a recent study suggests that FES may function both tumorigenic and tumor-suppressive effects (PMID: 28952025). Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag28095 Product name: Recombinant human FES protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 125-390 aa of BC035357 Sequence: QELTKTHSQDIEKLKSQYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHHQLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRERVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEH Predict reactive species Full Name: feline sarcoma oncogene Calculated Molecular Weight: 822 aa, 94 kDa Observed Molecular Weight: 93 kDa, 79 kDa, 87 kDa, 85 kDa GenBank Accession Number: BC035357 Gene Symbol: FES Gene ID (NCBI): 2242 RRID: AB_2881086 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P07332 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.