Proteintech, 28416-1-AP, CDKN2A/P16-INK4A Polyclonal antibody

Size: 20ul / 150ul The CDKN2A/P16-INK4A (28416-1-AP) by Proteintech is a Polyclonal antibody targeting CDKN2A/P16-INK4A in WB, ELISA applications with reactivity to mouse, rat samples 28416-1-AP targets CDKN2A/P16-INK4A in WB, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells, mouse uterus tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information p16INK4a is a pivotal protein encoded by the CDKN2A gene on human chromosome 9p21. It functions as a tumor suppressor and is one of the most important regulators of the cell cycle. It acts as a cyclin-dependent kinase (CDK) inhibitor, specifically binding to CDK4 and CDK6 and blocking their association with cyclin D. Beyond cell-cycle control, p16INK4a is a key mediator of cellular senescence-an irreversible growth-arrest state that limits the expansion of damaged or oncogene-expressing cells. Its expression rises with age and in response to stresses such as DNA damage or oncogenic signaling, making it a biomarker of both biological aging and tumorigenic stress. In human cancers, the p16INK4a-RB pathway is frequently disabled through CDKN2A deletion, mutation, or promoter hyper-methylation, underscoring its importance in tumor suppression. Specification Tested Reactivity: mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag28927 Product name: Recombinant mouse Cdkn2a protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-168 aa of NM_001040654 Sequence: MESAADRLARAAAQGRVHDVRALLEAGVSPNAPNSFGRTPIQVMMMGNVHVAALLLNYGADSNCEDPTTFSRPVHDAAREGFLDTLVVLHGSGARLDVRDAWGRLPLDLAQERGHQDIVRYLRSAGCSLCSAGWSLCTAGNVAQTDGHSFSSSTPRALELRGQSQEQS Predict reactive species Full Name: cyclin-dependent kinase inhibitor 2A Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 18-23 kDa GenBank Accession Number: NM_001040654 Gene Symbol: Cdkn2a Gene ID (NCBI): 12578 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P51480 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.