Proteintech, 28463-1-AP, F4/80 Polyclonal antibody

Size: 20ul / 150ul The F4/80 (28463-1-AP) by Proteintech is a Polyclonal antibody targeting F4/80 in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications with reactivity to mouse, rat samples 28463-1-AP targets F4/80 in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: unboiled J774A.1 cells, unboiled RAW 264.7 cells Positive IHC detected in: mouse spleen tissue, rat spleen tissue, rat liver tissue, mouse lung tissue, mouse heart tissue, mouse brain tissue, mouse liver tissue, mouse small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse spleen tissue, mouse liver tissue Positive IF-Fro detected in: mouse spleen tissue Positive IF/ICC detected in: RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:4000-1:16000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages. Specification Tested Reactivity: mouse, rat Cited Reactivity: human, mouse, rat, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag28252 Product name: Recombinant mouse Adgre1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 443-644 aa of NM_010130 Sequence: MELSKEETSTLGTILLETVESTMLAALLIPSGNASQMIQTEYLDIESKVINEECKENESINLAARGDKMNVGCFIIKESVSTGAPGVAFVSFAHMESVLNERFFEDGQSFRKLRMNSRVVGGTVTGEKKEDFSKPIIYTLQHIQPKQKSERPICVSWNTDVEDGRWTPSGCEIVEASETHTVCSCNRMANLAIIMASGELTME Predict reactive species Full Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1 Calculated Molecular Weight: 102 kDa Observed Molecular Weight: 130 kDa, 160 kDa GenBank Accession Number: NM_010130 Gene Symbol: F4/80 Gene ID (NCBI): 13733 RRID: AB_2881149 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q61549 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.