Proteintech, 28506-1-AP, Cytokeratin 5 Polyclonal antibody

Size: 20ul / 150ul The Cytokeratin 5 (28506-1-AP) by Proteintech is a Polyclonal antibody targeting Cytokeratin 5 in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 28506-1-AP targets Cytokeratin 5 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, mouse skin tissues, rat skin tissues Positive IHC detected in: human tonsillitis tissue, human brown disease, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human tonsillitis tissue Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag25436 Product name: Recombinant human KRT5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 6-60 aa of BC024292 Sequence: SVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGY Predict reactive species Full Name: keratin 5 Calculated Molecular Weight: 590 aa, 62 kDa Observed Molecular Weight: 62 kDa GenBank Accession Number: BC024292 Gene Symbol: Cytokeratin 5 Gene ID (NCBI): 3852 RRID: AB_2881159 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P13647 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.