Proteintech, 28590-1-AP, POLQ Polyclonal antibody

Size: 20ul / 150ul The POLQ (28590-1-AP) by Proteintech is a Polyclonal antibody targeting POLQ in WB, IF/ICC, ELISA applications with reactivity to human, rat samples 28590-1-AP targets POLQ in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, HuH-7 cells, HepG2 cells, L02 cells Positive IF/ICC detected in: A375 cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs). The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. Accumulating evidence showed that POLQ participates in the development and progression of human cancers because of its critical functions in the repair of genomic double-strand breaks (PMID: 34517891, 27264557). The POLQ cDNA encodes a polypeptide of 2592 amino acids with a predicted mass of 290 kDa, but POLQ is readily proteolyzed with smaller bands at approximately 70 kDa and 110 kDa (PMID: 17334634, 14576298). Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29643 Product name: Recombinant human POLQ protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 2342-2590 aa of NM_199420 Sequence: LSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV Predict reactive species Full Name: polymerase (DNA directed), theta Calculated Molecular Weight: 290 kDa Observed Molecular Weight: 250~290 kDa, 197 kDa GenBank Accession Number: NM_199420 Gene Symbol: POLQ Gene ID (NCBI): 10721 RRID: AB_3086067 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75417 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.