Proteintech, 28602-1-AP, LPL Polyclonal antibody

Size: 20ul / 150ul The LPL (28602-1-AP) by Proteintech is a Polyclonal antibody targeting LPL in WB, IHC, ELISA applications with reactivity to Human, mouse samples 28602-1-AP targets LPL in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples. Tested Applications Positive WB detected in: HeLa cells Positive IHC detected in: mouse brown adipose tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Lipoprotein lipase (LPL) is a key enzyme in lipid and lipoprotein metabolism. The canonical role of LPL involves the hydrolysis of triglyceride-rich lipoproteins for the provision of FFAs to metabolic tissues. LPL is highly expressed in adipocytes and myocytes as well as macrophages. Its protein level and activity are tightly regulated by multiple mechanisms in response to the metabolic state and energy demands of the cell . (PMID: 33277156, PMID: 33172164) Specification Tested Reactivity: Human, mouse Cited Reactivity: human, mouse, sheep Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: lipoprotein lipase Calculated Molecular Weight: 475 aa, 53 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC011353 Gene Symbol: LPL Gene ID (NCBI): 4023 RRID: AB_3086068 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P06858 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.