Proteintech, 28613-1-AP, CDC2L1 Polyclonal antibody

Size: 20ul / 150ul The CDC2L1 (28613-1-AP) by Proteintech is a Polyclonal antibody targeting CDC2L1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 28613-1-AP targets CDC2L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SKOV-3 cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HUVEC cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag28743 Product name: Recombinant human CDC2L1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-230 aa of NM_001787 Sequence: MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHRMEITIRNSPYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKDEKRKEKRRHRSHSAEGGKHARVKEKEREHERRKRHREEQDKARREWERQKRREMAREHSRRERDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMREDYSDKVKASH Predict reactive species Full Name: cell division cycle 2-like 1 (PITSLRE proteins) Calculated Molecular Weight: 93 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: NM_001787 Gene Symbol: CDC2L1 Gene ID (NCBI): 984 RRID: AB_2918181 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P21127 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.