Proteintech, 28692-1-AP, SETD3 Polyclonal antibody

Size: 20ul / 150ul The SETD3 (28692-1-AP) by Proteintech is a Polyclonal antibody targeting SETD3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 28692-1-AP targets SETD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells Positive IHC detected in: mouse small intestine tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells, SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SETD3 (SET domain protein 3), a ubiquitously expressed protein, is epigenetically up-regulated in response to muscle loading and hypertrophy. The SETD3 mRNA is ubiquitously expressed at a similar basal level in most examined tissues, with the noticeable exception of the skeletal muscle, kidneys, and testes (PMID: 34685411). SETD3 deficient female mice have severely decreased litter sizes due to primary maternal dystocia that is refractory to ecbolic induction agents (PMID: 30626964). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30017 Product name: Recombinant human SETD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-120 aa of NM_032233 Sequence: MGKKSRVKTQKSGTGATATVSPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRTLVEKIRKKQKGLSVTFDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELF Predict reactive species Full Name: SET domain containing 3 Calculated Molecular Weight: 67 kDa Observed Molecular Weight: 67 kDa GenBank Accession Number: NM_032233 Gene Symbol: SETD3 Gene ID (NCBI): 84193 RRID: AB_3086077 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86TU7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.