Proteintech, 28697-1-AP, LAMA1 Polyclonal antibody

Size: 20ul / 150ul The LAMA1 (28697-1-AP) by Proteintech is a Polyclonal antibody targeting LAMA1 in WB, IF-P, ELISA applications with reactivity to human, rat samples 28697-1-AP targets LAMA1 in WB, IF-P, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, U2OS cells Positive IF-P detected in: rat colon tissue Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information LAMA1 is also known as laminin subunit α1. Laminins are a family of glycoproteins found in the extracellular matrix that comprises the basement membrane. Laminins have a heterotrimeric structure composed of an α, β, and γ chain. Numerous biological processes are known to be directed by them, including cell adhesion, mitogenesis, differentiation, and metastasis, all of which contribute to carcinogenesis. Tissue distribution of LAMA1 occurred mainly in early epithelial development and some adult epithelia. Recent reports have shown that laminin-1 acts as an efficient attachment protein for a large variety of cultured cell types in vitro. (PMID: 36824663) Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag29329 Product name: Recombinant human LAMA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1592-1778 aa of NM_005559 Sequence: SNLENTTKYLQESLLKENMQKDLGKIKLEGVAEETDNLQKKLTRMLASTQKVNRATERIFKESQDLAIAIERLQMSITEIMEKTTLNQTLDEDFLLPNSTLQNMQQNGTSLLEIMQIRDFTQLHQNATLELKAAEDLLSQIQENYQKPLEELEVLKEAASHVLSKHNNELKAAEALVREAEAKMQES Predict reactive species Full Name: laminin, alpha 1 Calculated Molecular Weight: 337 kDa Observed Molecular Weight: 337 kDa GenBank Accession Number: NM_005559 Gene Symbol: LAMA1 Gene ID (NCBI): 284217 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P25391 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.