Proteintech, 28867-1-AP, SARS-CoV-2 S protein (944-1214 aa) Polyclonal antibody

Size: 20ul / 150ul The SARS-CoV-2 S protein (944-1214 aa) (28867-1-AP) by Proteintech is a Polyclonal antibody targeting SARS-CoV-2 S protein (944-1214 aa) in ELISA applications with reactivity to virus samples 28867-1-AP targets SARS-CoV-2 S protein (944-1214 aa) in WB, IF, ELISA applications and shows reactivity with virus samples. Tested Applications Positive ELISA detected in: Recombinant protein Recommended dilution Enzyme-linked Immunosorbent Assay (ELISA): ELISA : Background Information Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). This antibody detects the spike protein of SARS and SARS-COV-2. Specification Tested Reactivity: virus Cited Reactivity: human, monkey, hamster Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag30685 Product name: Recombinant virus spike protein protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 944-1214 aa of NC_045512 Sequence: ALGKLQDVVN QNAQALNTLV KQLSSNFGAI SSVLNDILSR LDKVEAEVQI DRLITGRLQS LQTYVTQQLI RAAEIRASAN LAATKMSECV LGQSKRVDFC GKGYHLMSFP QSAPHGVVFL HVTYVPAQEK NFTTAPAICH DGKAHFPREG VFVSNGTHWF VTQRNFYEPQ IITTDNTFVS GNCDVVIGIV NNTVYDPLQP ELDSFKEELD KYFKNHTSPD VDLGDISGIN ASVVNIQKEI DRLNEVAKNL NESLIDLQEL GKYEQYIKWP W Predict reactive species Full Name: SARS-CoV-2 Spike Protein Calculated Molecular Weight: 141 kDa GenBank Accession Number: NC_045512 Gene Symbol: SARS-COV-2 Gene ID (NCBI): 43740568 RRID: AB_2881223 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P0DTC2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.